Figure 2.

Panax notoginseng polysaccharides inhibited PRV infection in PK15 cells. PK15 cells were pretreated with 100, 200, 400, and 600 μg/mL Panax notoginseng polysaccharides for 1 h before being infected with PRV XJ5 (MOI = 0.1). Then, infected cells were cultured with 100, 200, 400, and 600 μg/mL Panax notoginseng polysaccharides. (A) The procedure for antiviral activity assay, adsorption assay, entry assay, and replication assay; (B) Infected cells were observed at 12 and 24 hpi; (C) At 24 hpi, gB protein was detected by Western blot; (D,E) Virus titer was evaluated by 50% tissue culture infective dose TCID₅₀ and plaque assay. All data are presented as means ± SD and were analyzed by GraphPad Prism software (GraphPad Software, San Diego, CA, USA) using one-way ANOVA (*** p = 0.002, **** p < 0.0001); (F) IFA for internalized virus was performed.