Skip to main content
. 2022 Feb 13;27(4):1254. doi: 10.3390/molecules27041254

Figure 3.

Figure 3

Panax notoginseng polysaccharides prevented pseudorabies virus (PRV) adsorption and entry to PK15 cells. PK15 cells were pretreated with 100, 200, 400, and 600 μg/mL Panax notoginseng polysaccharides for 1 h before cells were infected with PRV XJ5 (MOI = 0.1) at 4 °C for 1 h. Cells were incubated with 100, 200, 400, and 600 μg/mL Panax notoginseng polysaccharides at 37 °C for 1 h. (A) At 24 hpi, Western blot detected PRV gB protein expression; (B,C) viral titers were detected by TCID50 and plaque assay. All data are presented as means ± SD and were analyzed by GraphPad Prism software (GraphPad Software, San Diego, CA, USA) using one-way ANOVA (**** p < 0.0001); (D) IFA for internalized virus was performed.