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. 2022 Feb 13;27(4):1254. doi: 10.3390/molecules27041254

Figure 5.

Figure 5

Panax notoginseng polysaccharides did not prevent pseudorabies virus (PRV) entry to PK15 cells. PK15 cells were infected with PRV XJ5 (MOI = 0.1) at 4 °C for 1 h, and then infected cells were treated with 100, 200, 400, and 600 μg/mL Panax notoginseng polysaccharides for 1 h at 37 °C, before removal of the Panax notoginseng polysaccharides medium. Cells were cultured in Panax notoginseng polysaccharide-free 2% DMEM. (A) At 24 hpi, Western blot analysis detected PRV gB protein expression; (B,C) Viral titers were detected by TCID50 and plaque assay. All data are presented as means ± SD and were analyzed by GraphPad Prism software (GraphPad Software, San Diego, CA, USA) using one-way ANOVA (ns = not significant); (D) IFA for internalized virus was performed.