Figure 6.

Panax notoginseng polysaccharides inhibited pseudorabies virus (PRV) replication in PK15 cells. PK15 cells were infected with PRV XJ5 (MOI = 0.1) at 37 °C for 1 h, and then infected cells were incubated with 100, 200, 400, and 600 μg/mL Panax notoginseng polysaccharides until harvest. (A) At 6 hpi, cells were collected to detect the expression of PRV gB protein by Western blot analysis; (B) Viral titers were detected by TCID₅₀. All data are presented as means ± SD and were analyzed by GraphPad Prism software (GraphPad Software, San Diego, CA, USA) using one-way ANOVA (**** p < 0.0001); (C) IFA for internalized virus was performed.