TABLE 1.
In vitro digestion by QQR linker variants
| Substrate | Cleavagea by enzyme:
|
|||||
|---|---|---|---|---|---|---|
| L18 | L6 | L2 | L0 | L−3 | L−6 | |
| pQT4 | − | − | ± | ± | +++ | ++ |
| pQT5 | − | ++ | +++ | +++ | +++ | +++ |
| pQT6 | +++ | ++ | ++ | +++ | +++ | +++ |
| pQT7 | +++ | ++ | ++ | ++ | ± | − |
| pQT8 | +++ | ++ | + | ± | − | − |
| pQT10 | +++ | + | − | − | − | − |
| pQT12 | +++ | +++ | ++ | + | ± | − |
| pQT14 | +++ | +++ | ++ | +++ | +++ | +++ |
| pQT16 | +++ | +++ | ++ | +++ | +++ | ++ |
| pQT18 | +++ | +++ | ± | − | − | − |
| pQT20 | ++ | − | − | − | − | − |
| pQT26 | +++ | +++ | ++ | ++ | + | + |
| pQT30 | ++ | − | − | − | − | − |
| pQT35 | +++ | + | ± | − | − | − |
| pQT40 | − | |||||
a
Cleavage of the substrate DNAs was assessed semiquantitatively for each enzyme. The designations indicate the extent of cleavage at a molar ratio of nuclease to recognition sites of approximately 1:1. +++, 70 to 100% cleavage; ++, 20 to 50% cleavage; +, 5 to 10% cleavage; ±, barely detectable cleavage; −, no cleavage. pQT40 was tested only with L18.