Table 3.
Summary of publications covering consumption of ayahuasca decoction and concentration of individual substances derived from the decoction and their metabolites in human biological samples.
| Analytical Method | Matrix | Analyte and Concentration [ng/mL] | Calibration Range (ng/mL) | Year | Reference |
|---|---|---|---|---|---|
| LC-MS/MS | plasma | DMT [ND-15.1] DMT-NO [0.25–45.2] THH [25.9–55.4] THHOH [0.96–2.9] Harmine [0.54–5.2] Harmol [0.32–5.55] Harmaline [2.2–4.5] Harmalol [1.7–3.3] 2-MTHBC [ND-0.48] IAA [4.3–207.8] |
1–50 1–50 1–50 1–50 1–50 1–50 1–50 1–50 1–50 10–500 |
2012 | [20] |
| UHPLC-MS/MS | plasma | DMT [ND-4.3] THH [13.4–117.9] Harmaline [<LOQ-2.1] Harmine [<LOQ-40.6] |
1–150 2–150 1–150 1–150 |
2021 | [37] |
| LC-MS/MS | plasma | DMT [1.2–19.8] Harmine [1–15.6] Harmaline [2.7–15.7] THH [27.1–71.4] |
0.5–100 | 2012 | [38] |
| LC-MS/MS | plasma | DMT [2.5–65.9] NMT [0.57–7.6] DMT-NO [1.6–40.6] Harmine [5.9–511.5] Harmol [0–318.8] Harmaline [1.56–44.4] Harmalol [3.1–48.6] THH [22.6–895.7] THHOH [0–111] IAA [61.35–1674.6] |
1–50 1–50 1–50 1–50 1–50 1–50 |
2015 | [39] |
| 1–50 1–50 1–50 10–500 | |||||
| LC-MS/MS | serum | DMT [0.03–17.8] Harmine [0.1–61] Harmaline [0.1–5.4] THH [0.5–93.3] |
0.3–200 | 2021 | [40] |
| LC-MS/MS | serum | DMT [3.2] Harmine [12.3] Harmaline [0.3] THH [182] |
0.3–200 | 2017 | [41] |
| LC-MS/MS | urine | DMT [488] Harmine [60.6] Harmaline [49.4] THH [567] |
5–200 | 2017 | [41] |
| HPLC-MS/MS | urine | DMT [0.26–154.5] IAA [1152–5724] DMT-NO [155.2–2366] 2-MTHBC [8.08–19.4] NMT [0.52–6.12] Harmine [3.65–8.66] Harmaline [31.86–113.75] THH [307.7–1372.3] Harmol [56.2–146.1] Harmalol [121.9–193.4] THHOH [241.4–327.2] |
5–200 | 2012 | [21] |
| HPLC-MS/MS | urine | DMT [30–450] DMT-NO [1270–11,060] 2-MTHBC [10–130] 5-OH-DMT [140–160] Harmine [10–160] Harmaline [20–510] Harmol [40–3090] Harmalol [1250–4040] THH [10–6270] |
5–200 | 2011 | [25] |
| UHPLC-MS/MS | hair | DMT [5600] | 30–10,000 | 2014 | [42] |
| LC-MS/MS | Hair | DMT [3–1109] | 3–500 | 2021 | [43] |
| GC-MS | sweat | DMT [53–186.8 ng/patch] Harmine [319.8–1461.5 ng/patch] Harmaline [87.6–201.9 ng/patch] |
20–1500 ng/patch * | 2021 | [44] |
| LC-MS/MS | saliva | DMT [0.07–327.2] Harmine [0.01–95.2] Harmaline [0.08–131.3] THH [0.14–623.6] |
0.3–100 | 2021 | [40] |
* ng/patch is not possible to convert to ng/mL; ND-not detected; <LOQ-under quantification limit; HPLC-High-performance liquid chromatography; UHPLC-ultra high-performance liquid chromatography; GC-gas chromatography.