Table 1.
Comparison of L- and D-AvBD103b with other antimicrobial compounds, which we previously studied with the same technique (real-time fluorescence video microscopy, on living bacteria) and under the exact same conditions (30 °C, EZRDM buffer solution at pH 7.4).
| Antimicrobial Compound (Charge, Hydrophobicity) |
Observation of Periplasmic Bubbles (Duration) |
Event1: OM Permeabilization (GFP Leak to Surroundings) |
Event2: CM Permeabilization (Sytox Increase) |
Concomitance of Event 1 and Event 2 | Halt of Growth or Cell Shrinkage and Correlation with Event 1 |
Refs |
|---|---|---|---|---|---|---|
| LL-37 (+6, 35%) |
No | Very High | High | No | Cell shrinkage correlates with event 1 | [43] |
| Cecropin A (+6, 48%) |
No | Very High | Weak | No | Cell shrinkage correlates with event 1 | [52,84] |
| Melittin (+6, 46%) |
Yes (<24 s) |
High | High | Yes | Cell shrinkage correlates with event 1 | [45] |
| MM63:CHx37 (mean charge +22) |
Yes | Weak | High | No | Cell shrinkage precedes event 1 | [48,85] |
| L-AvBD103b (+10, 47%) |
Yes (minutes) |
Weak | Weak | Yes | Halt of growth precedes event 1 | This work |
| D-AvBD103b (+10, 47%) |
Yes (minutes) |
Weak | Weak | Yes | Halt of growth precedes event 1 | This work |