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. 2022 Feb 10;23(4):1961. doi: 10.3390/ijms23041961

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Vimentin interactome changes during metastatic transformation and upon inhibition of HDAC6 activity. Immortalised Bjhtert cells and metastasising BjhtertSV40TRasV12 cells without and with tubacin treatment. (A) Western blots for vimentin and actin in the cell fractions for the intermediate filament enrichment. (B,C) Mass spectrometry quantification of protein levels in the vimentin interactome in immortalised Bjhtert control cells and metastasising BjhtertSV40TRasV12 cells without (RasV12) and with (Tub) tubacin treatment. The six replicate measurements (two biological replicates, analysed in triplicate) are indicated by the coloured dots for each sample.