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. 2022 Feb 7;15(2):204. doi: 10.3390/ph15020204

Figure 3.

Figure 3

UPCDC-30245 inhibits the formation of early endosome and autophagy flux in H1299 and HeLa cells. (A,B) UPCDC-30245 (5 μM) inhibits the formation of EEA1 positive puncta after 1 h of treatment in H1299. 550 (DMSO), 528 (CB-5083) and 228 (UPCDC-30245) cells were counted from triplicates. Scale bar represents 25 μm. (C,D) The number of autophagosomes were increased and autolysosomes were reduced by 5 μM of UPCDC-30245, 50 μM of HCQ and 10 μM of Baf-A1, but not by 5 μM of CB-5083 in HeLa cells stably expressing mRFP-GFP-LC3 reporter after 2 h of treatment. Scale bar represents 50 μm. (E) UPCDC-30245 and HCQ induced the formation of large autophagosomes in HeLa cells after 2 h of treatment. The 210 (DMSO), 158 (CB-5083), 215 (UPCDC-30245), 225 (HCQ) and 291 (Baf-A1) cells were counted from four replicates. ns, not significant; ***, p < 0.0005; ****, p < 0.0001 according to one-way ANOVA with multiple comparison tests.