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. 2022 Feb 15;14(2):394. doi: 10.3390/v14020394

Figure 3.

Figure 3

ASFV K205R induces ER stress. (A,B) 3D4/21 (A) and HeLa (B) cells were transfected with K205R-HA plasmid as indicated for 24 h. Bip, p-PERK, PERK, p-eIF2α, eIF2α, ATF4, ATF6, XBP1, K205R-HA, and β-actin were assessed with immunoblotting analysis. (C–G) 3D4/21 cells were transfected with K205R-HA plasmid as indicated for 24 h. The mRNA levels of ERdj4 (C), Xbp1(s)/Xbp1(t) (D), Atf4 (E), Gadd34 (F), and Chop (G) were assessed with qRT-PCR analysis. * p < 0.05, ** p < 0.01, *** p < 0.001. (H) 3D4/21 cells were transfected with K205R-HA plasmid and treated with GSK2606414 (GSK, 10 μM) as indicated for 24 h. p-PERK, PERK, p-eIF2α, eIF2α, K205R-HA, and β-actin were assessed with immunoblotting analysis.