Table 4.

Overview of the MV and impurity contents of the final fraction. The MV suspensions in serum-containing and serum-free medium were concentrated (factor of 8) by UF/DF with a buffer exchange of five diavolumes. Data are means ± SEM (n = 5). Each run was evaluated separately and the data were combined to determine the overall recovery [37].

	Final Content
	MV in Serum-Containing Medium		MV in Serum-Free Medium
Infectious virus	3.2 × 107 ± 6.2 × 106 TCID50	49.3 ± 9.6%	7.4 × 106 ± 2.7 × 106 TCID50	54.5 ± 20.2%
Total viral RNA	1.4 × 1010 ± 1.6 × 109 copies mL−1	35.1 ± 4.1%	1.2 × 1010 ± 2.3 × 109 copies mL−1	19.9 ± 4.1%
hcDNA	369.2 ± 19.6 ng mL−1	23.3 ± 1.2%	19.1 ± 5.6 ng mL−1	2.0 ± 0.6%
Total protein	2882.1 ± 166.0 µg mL−1	7.1 ± 0.4%	29.4 ± 3.6 µg mL−1	0.7 ± 0.1%