FIG. 1.

Structure and gene expression of Idax. (A) Amino acid sequence of Idax. (B) Northern blot analysis of Idax in rat tissues. Total RNA (20 μg per lane) from the indicated rat tissues was probed with full-length cDNA of rat Idax. The positions of 18S and 28S rRNA are indicated. (C) Northern blot analysis of Idax in mouse embryos. Poly(A) RNA (2 μg per lane) from the indicated stages of mouse embryos was probed with the cDNAs of mouse β-actin and rat Idax-(369-495), which is identical with mouse Idax. E, embryonic day. (D) Schematic representation of deletion mutants of rat Idax and human Dvl-1 used in this study.