FIG. 4.

Subcellular localization of Idax, Dvl-1, and rAxin. L-Idax cells (A), wild-type L cells expressing GFP–Dvl-1 (B, C, and D), L-Idax cells expressing GFP–Dvl-1 (E, F, and G), wild-type L cells expressing Myc-rAxin (H), wild-type L cells coexpressing GFP–Dvl-1 and Myc-rAxin (I, J, and K), and L-Idax cells coexpressing GFP–Dvl-1 and Myc-rAxin (L, M, N, and O) were fixed and permeabilized. Some of them were directly viewed with a confocal laser-scanning microscope to detect GFP–Dvl-1 (B, E, I, and L), and the others were stained with the anti-HA antibody to detect HA-Idax (A, C, F, and O) or with the anti-Axin antibody to observe Myc-rAxin (H, J, and M). Note that nuclei were stained nonspecifically with the anti-HA antibody. Merged pictures of panels B and C, E and F, I and J, and L and M are shown in panels D, G, K, and N, respectively. GFP–Dvl-1, Cy5-labeled HA-Idax, and Alexa 546-labeled Myc-rAxin produced no cross staining. The results shown are representative of three independent experiments.