FIG. 7.

Effects of Idax on Wnt signal-dependent axis formation. (A) Effects of Idax on Wnt signal-dependent secondary axis formation. Embryos were injected ventrally with Xwnt-8 (40 pg) (a), Xwnt-8 and Idax (200 pg) (b), Dvl-1 (1 ng) (c), Dvl-1 and Idax (d), Xβ-catenin (1 ng) (e), or Xβ-catenin and Idax (f). Arrowheads in panel f indicate secondary axes. (B) The results from panel A are expressed as percentage of secondary axis formation. Black bars show complete axis duplication, which includes eyes and cement glands. White bars indicate incomplete axis duplication characterized by a lack of head structures but with a distinct branched axis. (C) Effects of Idax on Wnt-dependent rescue of axis formation. UV-treated embryos were injected with nothing (a), Idax (200 pg) (b), Xwnt-8 (40 pg) (c), Xwnt-8 and Idax (d), Xβ-catenin (1 ng) (e), or Xβ-catenin and Idax (f). (D) The results from panel C are expressed as DAI.