FIG. 2.

Expression and intracellular localization of CIA. (A, top) Northern blot of poly(A)⁺ mRNA from 17.5-day postcoitus mouse embryos, probed with the human CIA cDNA clone showing expression of a 3.7-kb mRNA in the heart, brain, lung, kidneys, and spleen. Note the presence of a second 3-kb mRNA in the kidneys. (A, bottom) A β-actin probe was used as control for mRNA integrity. Equal loading was assessed via ethidium bromide staining (not shown). (B) CIA is localized to the nucleus. Phase-contrast (i and iii) and fluorescence (ii and iv) pictures of HeLa cells transiently transfected with pGFP (i and ii) and pGFPhCIA (iii and iv) expression vectors.