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. 2022 Feb 25;8(8):eabl4386. doi: 10.1126/sciadv.abl4386

Fig. 2. Tsr2 promotes Rps26 release from mature 40S subunits in the presence of high salt in vitro.

Fig. 2.

Western blot analysis of pelleted (P) ribosomes and released proteins in the supernatant (S). Mature 40S subunits purified from yeast were incubated with (A) or without (C) recombinant Tsr2 under different salt concentrations. (B and D) Released Rps26 or Rps10 was quantified from (A) and (C) with two independent replicates for each condition. Error bars represent the SEM. (E) Rps26 release assay with the Rps26 interaction–deficient Tsr2_DWI mutant. (F) Quantification of Rps26 in (E) with two independent replicates for each condition. Error bars represent the SEM. (G) Rps26 release assay with the Tsr2 interaction–deficient Rps261–99 (lacking residues 100 to 119) truncation mutant. (H) Quantification of released Rps26 in (G) with two independent replicates for each condition. Error bars represent the SEM.