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. 2022 Feb 25;8(8):eabm4552. doi: 10.1126/sciadv.abm4552

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Fig. 6. — (A to C) Flow cytometry experiments with mGITR and no primary antibody (negative control) (A), mGITR with DTA-1 Fab (B), and mGITR with DTA-1 IgG (C). (D to F) Flow cytometry experiments with hGITR and no primary antibody (negative control) (D), hGITR with TRX518 Fab (E), and hGITR with TRX518 IgG (F). (G) Illustration of luciferase assay used to measure GITR activation by DTA-1 and TRX518 Fab and IgG. (H) Luciferase assay measuring mGITR activation in cells that are untreated or treated with DTA-1 Fab or DTA-1 IgG. (I) Luciferase assay measuring hGITR activation in cells that are untreated or treated with TRX518 Fab or TRX518 IgG. One-way analysis of variance (ANOVA) followed by a Tukey’s multiple comparison test was used to determine statistical significance between groups (****P < 0.0001).