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. 2022 Feb 5;14(2):365. doi: 10.3390/pharmaceutics14020365

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of arctiin on the S100A4 protein expression and mRNA levels of HeLa and SiHa cells. After being treated with arctiin at a concentration of 0, 10, 20, and 40 μM for 24 h, the S100A4 protein and mRNA expression of HeLa (A,C) and SiHa (B,D) were measured by Western blotting assay and real time PCR assay, respectively. After silencing S100A4 in the HeLa cells and SiHa cells, (E) Boyden chamber assay, (F) Boyden chamber invasion assay, and (G) Western blot were used for analysis. The values represented the means ± SD of at least 3 independent experiments. * p < 0.05, compared with the group that was without arctiin treatment.