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. 2022 Feb 14;18(2):e1010065. doi: 10.1371/journal.pgen.1010065

Fig 6. yonE is needed for production of infectious phage.

Fig 6

A. Strains carrying a temperature-sensitive SPß lysogen with a wild type yonE allele (black bars, CMJ114) or ΔyonE (white bars, CMJ455) were cultured in rich medium, then heat shocked for 20 minutes (methods). The Y axis shows the average and standard deviation of the OD600 from each culture immediately before and 70 minutes (± 5 minutes) after the heat shock. Each experiment was repeated ≥3 times. B. Phage were prepared by culturing strains with a temperature sensitive SPß lysogen with a wild type yonE allele (CMJ114), ΔyonE (CMJ455), or a ΔyonE allele with yonE complemented from the chromosome (lacA::{Pspank(hy)-yonE}, CMJ457) to an OD600 of approximately 0.4, and then heat shocking the cultures and collecting phage (methods). Lysates were then spread on lawns of the indicator strain CU1050 or an indicator with lacA::{Pspank(hy)-yonE} (CMJ440) and incubated overnight to allow plaque formation. The Y-axis shows the average and standard deviation of the number of infectious phage /ml, normalized by dividing by the OD600 of the culture at the time of heat shock. ND = not detected. Each experiment was repeated ≥3 times.