Table 1. B. subtilis strains used.
| B. subtilis Strain | Genotype (reference) |
|---|---|
| AG174 | trpC2 pheA1 a.k.a., JH642 [27] |
| CAL321 | trpC2 pheA1 Δ(rapI-yddM)318::kan [39] |
| CAL322 | trpC2 pheA1 Δ(yddG-yddM)319::kan [39] |
| CAL323 | trpC2 pheA1 Δ(yddB-yddM)320::kan [39] |
| CAL347 | trpC2 pheA1 Δ(ydcR-yddM)347::kan [39] |
| CAL348 | trpC2 pheA1 Δ(ydcQ-yddM)348::kan [39] |
| CAL1500 | trpC2 pheA1 Δ(spbK-rapI-phrI)1500::kan |
| CMJ48 | PY79 (ICEBs10) SPß+ amyE::{Pspank(hy)-empty lacI spc} |
| CMJ74 | trpC2 pheA1 ICEBs10 amyE::{spbK cat} |
| CMJ81 | CU1050 (SPß0) ICEBs1::kan (non-disruptive); note: made by crossing donor JMA448 with recipient CU1050 |
| CMJ82 | CU1050 (ICEBs10) (SPß0) amyE::{spbK cat} |
| CMJ98 | trpC2 pheA1 ICEBs10 yolBC98::spc |
| CMJ104 | PY79 (ICEBs10) SPß+ ΔyonF67::spc [42] |
| CMJ114 | CU1050 (ICEBs10) SPßc2 yolBC98::spc |
| CMJ116 | CU1050 (ICEBs10) SPßc2 yolBC98::spc amyE::{spbK cat} |
| CMJ388 | PY79 (ICEBs10) SPß+ ΔyonF155::spc (reverse orientation) [42] |
| CMJ402 | PY79 (ICEBs10) SPß+ ΔyonEF396::spc |
| CMJ403 | PY79 (ICEBs10) (SPß0) amyE::{Pspank(hy)-empty lacI spc} lacA::{Pspank(hy)-yonE lacI mls} |
| CMJ405 | PY79 (ICEBs10) (SPß0) amyE::{Pspank(hy)-empty lacI spc} lacA::{Pspank(hy)-empty lacI mls} |
| CMJ431 | trpC2 pheA1 amyE::{Pxyl-rapI xylR cat} Δ(spbK-rapI-phrI)1500::kan |
| CMJ434 | trpC2 pheA1 argA85::Tn917 SPßc2 ΔyonE434::lox-cat |
| CMJ440 | CU1050 (ICEBs10) (SPß0) lacA::{Pspank(hy)-yonE lacI mls} |
| CMJ443 | CU1050 (ICEBs10) SPßc2 ΔyonE443 (unmarked) |
| CMJ455 | CU1050 (ICEBs10) SPßc2 ΔyonE443 (unmarked) yolBC98::spc |
| CMJ457 | CU1050 (ICEBs10) SPßc2 ΔyonE443 (unmarked) yolBC98::spc lacA::{Pspank(hy)-yonE lacI mls} |
| CMJ472 | CU1050 (ICEBs10) (SPß0) comK::cat |
| CMJ616 | PY79 (ICEBs10) (SPß0) amyE::{Pspank(hy)-yonE lacI spc} |
| CMJ684 | PY79 (ICEBs10) (SPß0) lacA::{spbK kan} |
| CMJ685 | PY79 (ICEBs10) (SPß0) amyE::{Pspank(hy)-yonE lacI spc} lacA::{spbK kan} |
| CMJ826 | CU1050 SPßc2 yolBC98::spc ICEBs1::kan (non-disruptive) |
| CMJ827 | CU1050 (SPß0) ICEBs1::kan, comK::cat |
| CMJ913 | CU1050 (SPß0) ICEBs1+ Δ(rapI-phrI)342::kan |
| CMJ914 | CU1050 (SPß0) ICEBs1+ Δ(spbK-rapI-phrI)1500::kan |
| CMJ917 | CU1050 SPßc2 ICEBs1+ Δ(rapI-phrI)342::kan, yolBC98::spc |
| CMJ918 | CU1050 SPßc2 ICEBs1+ Δ(spbK-rapI-phrI)1500::kan, yolBC98::spc |
| CU1050 | ICEBs10 SPß0 metA thrC leu codY sup-3 (trnS-lys) [31,33]; (CMJ28) |
| IRN342 | trpC2 pheA1 Δ(rapI-phrI)342::kan [22] |
| JMA222 | trpC2 pheA1 ICEBs10 [22] |
| JMA448 | trpC2 pheA1 ICEBs1::kan amyE::{Pspank(hy)-rapI spc} [22] note: used as donor with recipient CU1050 to create CMJ81 |
| KM250 | trpC2 pheA1 Δ(rapI-phrI)342::kan amyE::{Pxyl-rapI cat} [61] |
| PY79 | ICEBs10 SPß0 [62] |