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. 2022 Feb 25;17(2):e0264543. doi: 10.1371/journal.pone.0264543

Fig 2. Results from KASP assay for SNPs on Ppe.XapF6 using clean DNA extraction and previously genotyped samples.

Fig 2

Solid yellow shapes indicate positive controls, and empty shapes indicate unknowns, with the shape indicating the assigned genotype as follows: circle = no template/no amplification, triangle = AA, diamond = AB, square = BB. Dashed lines in plots in the top row indicate boundaries for no amplification (% fluorescence < ~20% for both fluorophores). Dashed lines in plots in the bottom row indicate delta (% HEX—% FAM) values separating heterozygous and homozygous genotypes, adjusted to reflect assay conditions. Points are automatically encircled in red when they are close enough to the dashed lines in the bottom row that manual inspection is advised. A,B = F6-2; C,D = F6-3; E,F = F6-4.