Fig 4. Results from KASP assay for two SNPs, Ppe.Xap F1-1 and Ppe.Xap F6-2, using endpoint PCR with crude DNA extracts from greenhouse-grown seedling samples.

Solid yellow shapes indicate positive controls, solid red indicates an unknown that failed to amplify, and empty shapes indicate unknowns, with the shape indicating the assigned genotype as follows: circle = no template/no amplification, triangle = AA, diamond = AB, square = BB. Dashed lines in plots in the top row indicate boundaries for no amplification (% fluorescence < ~20% for both fluorophores). Dashed lines in plots in the bottom row indicate delta (% HEX—% FAM) values separating heterozygous and homozygous genotypes, adjusted to reflect assay conditions.