FIG. 5.

(A-D). (A) Bioinformatics analysis of matching sequence of miR-613 within CircCCT3. MUT CircCCT3 is the mutation of the match sequence of CircCCT3 with miR-613. (B) The luciferase reporter assay revealed that miR-613 binds to the CircCCT3, not MUT CircCCT3. (C). Bioinformatics analysis of matching sequence of miR-613 within 3'-UTR of VEGFA. MUT VEGFA 3'-UTR is the mutation of the match sequence of 3'-UTR of VEGFA with miR-613. (D) The luciferase reporter assay revealed that miR-613 binds to the 3'-UTR of WT VEGFA, not MUT VEGFA. Relative luciferase activity was quantified and the data were demonstrated as mean ± SD. *P < .05 vs. respective NC groups; NC, negative control; WT, wild-type; MUT, mutant.