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. 2022 Feb 4;11:e60183. doi: 10.7554/eLife.60183

Figure 7. Synergistic action of recombinant Dll4(E12) and parathyroid hormone (PTH).

(A) Representative micro-computed tomography (μ-CT) images of trabecular bone in the distal femoral metaphysis of female wild-type mice treated with vehicle, pLIVE-Dll4(E12), PTH, or the combination of both for 3 weeks. (B–G) Quantitative analysis of μ-CT data on trabecular bone volume/total volume (B), trabecular connectivity density (C), trabecular number (D), bone surface (E), trabecular thickness (F), trabecular separation (G) in the distal femoral metaphysis. Data represent mean ± SD. (n = 5 mice) (p-values determined by one-way ANOVAs (F, G) followed by Sidak’s multiple comparisons test or Brown-Forsythe and Welch’s ANOVAs (B, C, D, E) followed by Dunnett’s T3 multiple comparisons test). (H) Representative μ-CT images of cortical bone in the female midfemoral diaphysis. (I–K) Graphs from μ-CT analysis of the cortical bone volume/total volume (I), cortical thickness (J), and cortical porosity (K) in the midfemoral diaphysis. Data represent mean ± SD. (n = 5 mice) (p-values determined using one-way ANOVAs followed by Sidak’s multiple comparisons test).

Figure 7—source data 1. Source data for Figure 7B–G,I-K.

Figure 7.

Figure 7—figure supplement 1. Effects of combined parathyroid hormone (PTH) and Dll4(E12) administration.

Figure 7—figure supplement 1.

(A) Time course of pLIVE-Dll4(E12) and PTH administration to adult wild-type female mice. (B–E) Representative overview confocal images and corresponding quantification of immunostainings for Dll4 (B, green), Collagen I alpha one chain (Col1a1) (C, green), PDGFRβ (D, green), Endomucin (E, red) in the femurs treated with vehicle, pLIVE-Dll4(E12), PTH, or the combination of both. Nuclei, DAPI (blue). Data represent mean ± SD. (n = 4–5 mice). Statistical significance was assessed using one-way ANOVAs (B, C, D) followed by Sidak’s multiple comparisons test or Brown-Forsythe and Welch’s ANOVA (E) followed by Dunnett’s T3 multiple comparisons test.
Figure 7—figure supplement 1—source data 1. Source data for Figure 7—figure supplement 1B-E.
Figure 7—figure supplement 2. Synergistic effects of Dll4(E12) and parathyroid hormone (PTH) on osteogenesis.

Figure 7—figure supplement 2.

Representative overview confocal images and corresponding quantifications for Osterix (OSX) (A, white) and Opn (B, green) immunostaining in wild-type female femur after treatment with vehicle, pLIVE-Dll4(E12), PTH, or the combination of both. Nuclei, DAPI (blue). Data represent mean ± SD. (n = 5 mice) (p-values determined using one-way ANOVA followed by Sidak’s multiple comparisons test).
Figure 7—figure supplement 2—source data 1. Source data for Figure 7—figure supplement 2A,B.