| Strain, strain background (Mus musculus, C57BL/6JRj) |
WT |
Janvier Labs |
|
|
| Genetic reagent (Mus musculus) |
Tg(Hey1-EGFP)ID40Gsat
|
GENSAT |
MGI:4847129 |
|
| Recombinant DNA reagent |
pcDNA3.1- Dll4(ECD)- His(6x)-Asp(8x)(plasmid) |
This paper |
|
Dll4-Asp(8x)
|
| Recombinant DNA reagent |
pcDNA3.1- Dll4(ECD)- Variant- His(6x)-Asp(8x) (plasmid) |
This paper |
|
Dll4(E12)
|
| Recombinant DNA reagent |
pLIVE-Dll4(ECD)-His(6x)-Asp(8x) (plasmid) |
This paper |
|
pLIVE-Dll4-Asp(8x) |
| Recombinant DNA reagent |
pLIVE-Dll4(ECD) Variant- His(6x)-Asp(8x) (plasmid) |
This paper |
|
pLIVE-Dll4(E12) |
| Recombinant DNA reagent |
pcDNA3.1- Jag1 (ECD)- His(6x)-Asp(8x)(plasmid) |
This paper |
|
Jag1- Asp(8x)
|
| Recombinant DNA reagent |
pcDNA3.1- Jag1 (ECD)- JV1- His(6x)-Asp(8x)(plasmid) |
This paper |
|
Jag1(JV1)
|
| Recombinant DNA reagent |
pLIVE- Jag1 (ECD)- His(6x)-Asp(8x) (plasmid) |
This paper |
|
pLIVE-Jag1- Asp(8x) |
| Recombinant DNA reagent |
pLIVE- Jag1 (ECD)- JV1- His(6x)-Asp(8x) (plasmid) |
This paper |
|
pLIVE-Jag1(JV1) |
| Cell line (Homo sapiens) |
Human Umbilical Vein Endothelial Cells (HUVEC) |
ThermoFisher |
Cat# C0035C |
Cell identity and absence of mycoplasma contamination or human pathogens were certified by the supplier |
| Cell line (Homo sapiens) |
Human Embryonal kidney –293(HEK293) |
DSMZ |
Cat# ACC 305 |
Cell identity and absence of mycoplasma contamination were certified by the supplier |
| Antibody |
Anti-Endomucin (Rat, monoclonal) |
Santa Cruz |
Cat# SC-65495 |
IF (1:100) |
| Antibody |
Anti-PECAM-1(Rat, monoclonal) |
Pharmigen |
Cat# 553,370 |
IF (1:100) |
| Antibody |
Anti-CD31(Goat, polyclonal) |
R&D Systems |
Cat# FAB3628 |
IF (1:100) |
| Antibody |
Anti-Pdgfrβ (Goat, polyclonal) |
R&D Systems |
Cat# AF1042 |
IF (1:100) |
| Antibody |
Anti-NG2 (Rabbit, polyclonal) |
Millipore |
Cat# AB5320 |
IF (1:100) |
| Antibody |
Anti-BCAM (Goat, polyclonal) |
R&D Systems |
Cat# AF8299 |
IF (1:50) |
| Antibody |
Anti-ATP6V1B1+ ATP6V1B2 (Rabbit, polyclonal) |
Abcam |
Cat# ab200839 |
IF (1:100) |
| Antibody |
Anti-Aggrecan (Rabbit, polyclonal) |
Millipore |
Cat# AB1031 |
IF (1:100) |
| Antibody |
Anti-Sox9 (Goat, polyclonal) |
R&D Systems |
Cat# AF3075 |
IF (1:100) |
| Antibody |
Anti-Perilipin (Rabbit, polyclonal) |
Cell Signaling |
Cat# 9349 |
IF (1:100) |
| Antibody |
Anti-Osterix (Rabbit, polyclonal) |
Abcam |
Cat# ab22552 |
IF (1:1000) |
| Antibody |
Anti-Collagen Type I (Rabbit, polyclonal) |
Millipore |
Cat# AB765P |
IF (1:200) |
| Antibody |
Anti-Osteopontin (Goat, polyclonal) |
R&D Systems |
Cat# AF808 |
IF (1:100) |
| Antibody |
Anti-Osteocalcin (Rabbit, polyclonal) |
LifeSpan BioSciences |
Cat# LS-C17044
|
IF (1:100) |
| Antibody |
Anti-Runx2 (Rabbit, monoclonal) |
R&D Systems |
Cat# MAB2006 |
IF (1:50) |
| Antibody |
Anti-Dll4 (Goat, polyclonal) |
R&D Systems |
Cat# AF1389 |
IF (1:100)WB (1:200) |
| Antibody |
Anti-Jag1 (Goat, polyclonal) |
Sigma |
Cat# J4127 |
IF (1:100)WB (1:500) |
| Antibody |
Anti-GAPDH (Rabbit, monoclonal) |
Ambion |
Cat# AM4300 |
WB (1:1000) |
| Antibody |
Anti-CD45-FITC (Rat, monoclonal) |
eBioscience |
Cat# 11–0451 |
|
| Antibody |
Anti-CD8-Biotin (Rat, monoclonal) |
eBioscience |
Cat# 13–0081 |
|
| Antibody |
Anti-CD4-APC (Rat, monoclonal) |
eBioscience |
Cat# 17-0042-82 |
|
| Antibody |
Streptavidin PE/Cy7 |
Thermo Scientific |
Cat# SA1012 |
|
| Antibody |
Lineage Cell Depletion Kit |
Miltenyi Biotec |
Cat# 130-090-858 |
|
| Antibody |
CD45 Microbeads |
Miltenyi Biotec |
Cat# 130-052-301 |
|
| Antibody |
CD117 Microbeads |
Miltenyi Biotec |
Cat# 130-091-224 |
|
| Antibody |
Ter-119 Microbeads |
Miltenyi Biotec |
Cat# 130-049-901 |
|
| Antibody |
Anti-rabbit Alexa Fluor-488 (Donkey, polyclonal) |
Invitrogen |
Cat# A21206 |
IF (1:500) |
| Antibody |
Anti-rabbit Alexa Fluor-594 (Donkey, polyclonal) |
Invitrogen |
Cat# A21207 |
IF (1:500) |
| Antibody |
Anti-rabbit Alexa Fluor-647 (Donkey, polyclonal) |
Invitrogen |
Cat# A31573
|
IF (1:500) |
| Antibody |
Anti-rat Alexa Fluor-488 (Donkey, polyclonal) |
Invitrogen |
Cat# A21208 |
IF (1:500) |
| Antibody |
Anti-rat Alexa Fluor-594 (Donkey, polyclonal) |
Invitrogen |
Cat# A21209 |
IF (1:500) |
| Antibody |
Anti-rat Alexa Fluor-647 (Donkey, polyclonal) |
Jackson ImmunoResearch |
Cat# 712-605-153 |
IF (1:500) |
| Antibody |
Anti-rat Alexa Fluor-647 (Donkey, polyclonal) |
Invitrogen |
Cat# A21247 |
IF (1:500) |
| Antibody |
Anti-goat Alexa Fluor-488 (Donkey, polyclonal) |
Invitrogen |
Cat# A11055 |
IF (1:500) |
| Antibody |
Anti-goat Alexa Fluor-647 (Donkey, polyclonal) |
Invitrogen |
Cat# A21447 |
IF (1:500) |
| Antibody |
Anti-goat Alexa Fluor-647 (Donkey, polyclonal) |
Thermo Scientific |
Cat# A32849 |
IF (1:500) |
| Antibody |
Anti-rabbit IgG HRP-linked (Goat) |
Cell Signaling |
Cat# 7074 |
WB (1:15000) |
| Antibody |
Anti-goat IgG HRP-linked (Donkey) |
Antibody online |
Cat# ABIN1536502 |
WB (1:15000) |
| Antibody |
Anti-mouse IgG 656G HRP-linked (Sheep) |
GE Healthcare |
Cat# NA931 |
WB (1:40000) |
| Antibody |
Anti-goat IgG (H + L) Peroxidase AffiniPure Bovine |
Jackson ImmunoResearch |
Cat# 805-035-180 |
WB (1:15000) |
| Sequence-based reagent |
Human GAPDH Endogenous Control (VIC/MGB probe, primer limited) |
Applied Biosystems |
Cat# 4326317E |
TaqMan probe Hs99999905_m1 |
| Sequence-based reagent |
Human ACTB Endogenous Control (VIC/MGB probe, primer limited) |
Applied Biosystems |
Cat# 4326315E |
TaqMan probe Hs99999903_m1 |
| Sequence-based reagent |
Human HEY1 TaqMan Gene Expression Assay (FAM) |
Applied Biosystems |
Cat# 4331182 |
TaqMan probe Hs00232618_m1 |
| Sequence-based reagent |
Human DLL4 TaqMan Gene Expression Assay (FAM) |
Applied Biosystems |
Cat# 4331182 |
TaqMan probe Hs00184092_m1 |
| Sequence-based reagent |
Human HEY2 TaqMan Gene Expression Assay (FAM) |
Applied Biosystems |
Cat# 4331182 |
TaqMan probe Hs00232622_m1 |
| Sequence-based reagent |
Human HES1 TaqMan Gene Expression Assay (FAM) |
Applied Biosystems |
Cat# 4331182 |
TaqMan probe Hs00172878_m1 |
| Sequence-based reagent |
Human EFNB2 TaqMan Gene Expression Assay (FAM) |
Applied Biosystems |
Cat# 4331182 |
TaqMan probe Hs00187950_m1 |
| Sequence-based reagent |
Human NRARP TaqMan Gene Expression Assay (FAM) |
Applied Biosystems |
Cat# 4331182 |
TaqMan probe Hs01104102_s1 |
| Peptide, recombinant protein |
Recombinant Human PTH (1-34) |
BACHEM |
Cat# H-4835-GMP, 4033364 |
|
| Commercial assay or kit |
BCA Protein Assay Kit |
Pierce |
Cat# 23225 |
|
| Commercial assay or kit |
RNeasy Plus Micro Kit |
QIAGEN |
Cat# 74034 |
|
| Commercial assay or kit |
iScript cDNA Synthesis Kit |
BIO-RAD |
Cat# 170–8891 |
|
| Commercial assay or kit |
SsoAdvanced Universal Probes Supermix |
BIO-RAD |
Cat# 172–5284 |
|
| Commercial assay or kit |
LEGENDplex Mouse Inflammation Panel (13-plex) with V-bottom plates |
BioLegend |
Cat# 740446 |
|
| Commercial assay or kit |
Anticoagulant EDTA-treated Microvettes |
Sarstedt |
Cat# 20.1341 |
|
| Commercial assay or kit |
BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification kit |
BD Biosciences |
Cat# 633,801 |
|
| Commercial assay or kit |
Agencourt AMPure XP magnetic beads |
Beckman Coulter Life Sciences |
Cat# A638880 |
|
| Commercial assay or kit |
Disposable polystyrene columns |
Thermo Scientific |
Cat# 29922 |
|
| Commercial assay or kit |
Amicon Ultra-0.5 Centrifugal Filter |
Millipore |
Cat# UFC500396 |
|
| Commercial assay or kit |
Pierce Slide-A-Lyzer 10K MWCO Dialysis Cassettes |
Thermo Scientific |
Cat# 66380 |
|
| Chemical compound, drug |
Ni-NTA agarose resin |
Qiagen |
Cat# 302010 |
|
| Chemical compound, drug |
Sucrose |
Sigma |
Cat# S0389 |
|
| Chemical compound, drug |
cOmplete ULTRA Tablets Protease Inhibitor Cocktail |
Roche |
Cat# 05892970001 |
|
| Chemical compound, drug |
Phosphatase inhibitor cocktail set V |
EMD Millipore |
Cat# 524629 |
|
| Chemical compound, drug |
SimplyBlue SafeStain |
Invitrogen |
Cat# LC6060 |
|
| Chemical compound, drug |
Gelatine |
Sigma |
Cat# G1890 |
|
| Chemical compound, drug |
Polyvinylpyrrolidone |
Sigma |
Cat# P5288 |
|
| Chemical compound, drug |
Trypsin-EDTA solution |
Sigma |
Cat# T3924 |
|
| Chemical compound, drug |
Paraformaldehyde |
Sigma |
Cat# P6148 |
|
| Chemical compound, drug |
70 kDa Dextran, Texas Red Lysine fixable |
Molecular Probes |
Cat# D1864 |
|
| Chemical compound, drug |
Calcein |
Sigma |
Cat# C0875 |
|
| Chemical compound, drug |
Fluoromount-G |
Southern Biotech |
Cat# 0100–01 |
|
| Chemical compound, drug |
ECL Prime Western Blotting Detection Reagent |
GE-Healthcare |
Cat# RPN2236 |
|
| Chemical compound, drug |
4-Hydroxy tamoxifen |
Sigma |
Cat# H7904 |
|
| Chemical compound, drug |
Hematoxilin |
Sigma |
Cat# MHS16 |
|
| Chemical compound, drug |
Dimethyl sulfoxide |
Sigma |
Cat# D8418 |
|
| Chemical compound, drug |
MEM200 endothelial cells medium |
ThermoFisher |
Cat# M200500 |
|
| Chemical compound, drug |
LSGS |
ThermoFisher |
Cat# S00310 |
|
| Chemical compound, drug |
EBM-2 endothelial cells medium |
Lonza |
Cat# CC-3156 |
|
| Chemical compound, drug |
EGM-2 Single Quots |
Lonza |
Cat# CC-4176 |
|
| Chemical compound, drug |
Opti-MEM |
Gibco |
Cat# 31985–047 |
|
| Chemical compound, drug |
Poly-L-lysine |
Sigma |
Cat# P6282 |
|
| Chemical compound, drug |
HEPES |
Sigma |
Cat# H3537 |
|
| Chemical compound, drug |
Ketamine |
Zoetis |
Cat# 344771 |
|
| Chemical compound, drug |
Rompum |
Bayer HealthCare |
Cat# D-51368 |
|
| Chemical compound, drug |
Collagenase I |
Gibco |
Cat# 17100–017 |
|
| Chemical compound, drug |
Collagenase IV |
Gibco |
Cat# 17104–019 |
|
| Chemical compound, drug |
Dispase |
Gibco |
Cat# 17105–041 |
|
| Software, algorithm |
ImageJ (v2.0.0 Fiji) |
Schindelin et al., 2012
|
|
|
| Software, algorithm |
Volocity (v6.3) |
Perkin Elmer |
|
|
| Software, algorithm |
Illustrator (vCC2018) |
Adobe |
|
|
| Software, algorithm |
GraphPad Prism7 |
GraphPad Software |
|
|
| Software, algorithm |
FlowJo (v10.3) |
BD Life Sciences |
|
|
| Other |
DAPI stain |
Sigma |
Cat# D9542 |
(1 mg/mL) |
