Figure 3.

Low-dose and long-term expression of Cr(VI)-induced mitochondria dysfunction. After L02 cells were treated with Cr(VI) for 4 weeks, (a) the TMRM (red) staining assay for the detection of ΔΨm, and Mito-Tracker Green (green) was used to locate the mitochondria (200x). (b) ATP content, (c) mitochondria mass, and (d) mtDNA copy number were also detected. (e) Mitochondrial morphology was determined by specific fluorescence probe Mito-Tracker Red CMXRos (red) (200x). All experiments were repeated at least 3 times and expressed as mean ± SD. ^∗p < 0.05, compared with control; ^#p < 0.05, compared with Cr(VI)-exposed group.