Figure 8.

Active NF-κB potentiates premature senescence without influencing intracellular Ca²⁺ concentration. PDTC was used to pretreat the L02 hepatocytes before Cr(VI) exposure for 4 weeks. (a) p65 protein (cytoplasm and nucleus) levels were detected by Western blot, and ImageJ software was used to analyze the relative levels of proteins normalized to the expression of GAPDH. (b) β-Galactosidase Staining Kit (200x) and (c) DAPI staining were used to detect SAHF (200x). (d) IL-6 and FGF23 secretion was analyzed by ELISA kit. (e) The fluorescence of intracellular and mitochondria Ca²⁺was quantitated via spectrofluorometry. All experiments were repeated at least 3 times and expressed as mean ± SD. ^∗p < 0.05, compared with control; ^#p < 0.05, compared with Cr(VI)-exposed group.