Fig. 3. The effect of Bok in murine NSCLC is dependent on functional p53.

a Representative images of H&E staining of lungs of the indicated genotypes 13 weeks after infection with 5 × 10⁶ PFU of AdenoCre. Lower panels are higher magnification of indicated areas. Scale bars represent 1 mm or 100 µm, respectively. b Quantification of the H&E staining expressed as the ratio between the area of the lesions over the total lung area. Data were analyzed by one-way ANOVA (p = 0.9654, F(2,20) = 0.0325). c Quantification of the H&E staining expressed as number of lesions per mm². Data were analyzed by one-way ANOVA (p = 0.5803, F(2,20) = 0.5593). d, e Representative images, and quantification of Ki67 staining. Data are reported as number of positive cells normalized to lesion area. Data were analyzed by Kruskal–Wallis test (p = 0.1013, H(3) = 4.579). f Quantification of γ-H2AX staining. Data are reported as percentage of positive staining normalized to lesion area. Data were analyzed by Kruskal–Wallis test (p = 0.0783, H(3) = 5.094). For the quantifications reported in panel (b, c), three sections per animal, separated by 100 µm, were analyzed. For the quantification in panel (e) and (f), six lesion per slide/animal were evaluated. n = number of animals. Data in panel (b, c, e, and f) are presented as dot plot and report mean ± SEM.