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. 2022 Jan 29;41(9):1376–1382. doi: 10.1038/s41388-021-02161-1

Fig. 4. Bok-deficiency sensitizes against DNA damaging agents.

Fig. 4

a DNA damage in Etoposide-treated LKR10 LacZ and Bok−/− cells. Cells were treated with 6 µg/ml Etoposide for indicated times and the level of DNA damage (% DNA in tail) analyzed by alkaline comet assay. Percent tail in DNA (% DNA in tail as measured in comet assay) after exposure to Etoposide (left panel). Representative microscopic images of the comet assay (right panel). Data are means ± SEM of at least 25 cells per group analyzed across two slides. Statistical differences at the indicated times were determined by unpaired t-test with Welch correction. b Cell viability of LKR10 LacZ and Bok−/− cells treated with Etoposide (4 µg/ml) or Irinotecan (80 µM) for indicated times. Data are means ± SEM of three independent experiments. Significant differences from LacZ were revealed by two-way ANOVA, Bonferroni’s multiple comparison test (*: P < 0.05; **: P < 0.01; ***: P < 0.001).