Fig. 3.

SPINT2 modulates the expression of p21 independent of p53 level. A Expression levels of p53 and p21 upon depletion of the candidate factors in DCD treated HCT116 cells. Ponceau staining was used as a loading control. B, C Quantification of p53 and p21 levels upon depletion of the candidate factors in DCD treated cells. Mean and SEM of three experiments are shown. T test was used for statistical evaluation. D Schematic presentation of the experiment and expression of p53 and p21 in doxorubicin (DOX) treated cells depleted for SPINT2. Ponceau staining was used as a loading control. E Quantification of p53 and p21 levels upon SPINT2 depletion in DOX treated cells. Mean and SEM are shown. T test was used for statistical evaluation. F Normalized mRNA levels of p53 downstream targets upon SPINT2 depletion in DOX treated cells. Mean and SEM of at least three independent experiments are shown, normalized to control RPL30. G Immunoblots of p53 and p21 in RPE1, DLD1 and CaCO2 cells upon SPINT2 depletion. Ponceau staining was used as a loading control. H Correlation of CDKN1A and SPINT2 mRNA expression levels in colorectal adenocarcinoma and in normal tissues. RSEM (RNA-Seq by Expectation Maximization) quantification of RNA abundance derived from RNA-seq data