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. 2021 Dec 28;45(1):103–119. doi: 10.1007/s13402-021-00654-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — USP28 affects centrosome clustering in proliferating tetraploids. A Volcano plot showing the interaction partners of USP28 (blue) in diploid and tetraploid HCT116 cells. The interactors NuMA1 and MDC1 are marked in green. Complete results are provided in Supplementary Table 4. B Immunoblot of USP28 and NuMA1 after co-immunoprecipitation of USP28 using magnetic beads (upper panel). Ratio between NuMA1 and respective whole-cell lysates (lower panel). Samples were treated with DCD and aphidicolin (Aph), respectively. C Schematics of the experiment and immunofluorescence pictures in HCT116 γ-tubulin-mRuby cells stained for USP28 and NuMA1 showing localization of USP28 and NuMA1 in relation to γ-tubulin. DNA was stained using DAPI. Scale bars: 10 µm. D Percentage of pseudo-bipolar spindles in tetraploid HCT116 γ-tubulin-mRuby cells and after USP28 siRNA knockdown. Cells were fixed 24 h after DCD washout and analyzed by immunofluorescence. T test was used for statistical evaluation. E Analysis of centrosome numbers in tetraploid HCT116 γ-tubulin-mRuby cells and after USP28 siRNA knockdown. T test was used for statistical evaluation