Table 5.
Spatial analysis of adaptive immune cells and the proportions of “conventional” cells participating in the foreign body reaction using Euclidean distance maps
| Parameter (n = 7 PP meshes) | Distance from mesh fibers | |||||
|---|---|---|---|---|---|---|
| Zone1 000–050 µm |
Zone2 050–100 µm |
Zone3 100–150 µm |
Zone4 150–200 µm |
Zone5 200–250 µm |
Zone6 250–350 µm |
|
| Cell density (cells/mm2) | 4,418.2 (80.2) | 3,602.8 (98.6)a | 2,849.3 (95.2) | 2,386.1 (89.7) | 2,018.9 (91.8) | 1,696.1 (108.4)b |
| % CD3+ | 19.3 (3.5) | 21.6 (2.8) | 15.4 (2.5) | 11.3 (2.3) | 9.5 (2.2) | 8.8 (2.0)b |
|
% CD3+CD68−/CD3+ “conventional” |
47.8 (9.1) | 70.8 (8.4) | 73.3 (8.1) | 75.0 (6.3) | 73.0 (7.0) | 70.3 (6.0) |
| % CD4+ | 28.8 (3.9) | 19.8 (4.0) | 16.5 (3.7) | 14.4 (3.5) | 13.9 (3.1) | 12.6 (2.8)b |
|
% CD4+CD68−/CD4+ “conventional” |
20.7 (4.1) | 50.5 (7.9)a | 54.6 (6.5) | 56.2 (5.6) | 58.4 (5.5) | 57.5 (7.4)b |
| % CD8+ | 16.7 (5.5) | 12.1 (2.0) | 9.0 (1.7) | 8.1 (2.2) | 6.5 (1.6) | 4.8 (0.9)b |
|
% CD8+CD68−/CD8+ “conventional” |
44.0 (8.1) | 79.3 (4.2)a | 83.6 (3.4) | 78.3 (4.6) | 72.2 (4.9) | 77.5 (4.4)b |
| % CD20+ | 1.7 (0.9) | 2.2 (1.3) | 2.5 (1.5) | 3.2 (2.0) | 3.1 (1.7) | 3.3 (1.9) |
|
% CD20+CD68−/CD20+ “conventional” |
57.3 (12.1) | 91.8 (6.8) | 92.4 (3.0) | 95.3 (2.8) | 85.2 (10.8) | 95.1 (2.5) |
| % FoxP3+ | 32.4 (4.4) | 22.2 (3.1) | 18.6 (3.6) | 17.5 (4.2) | 16.5 (4.1) | 15.6 (4.3)b |
|
% FoxP3+CD68−/FoxP3+ “conventional” |
33.5 (1.5) | 67.6 (3.9)a | 79.9 (3.4) | 83.7 (3.3) | 87.3 (2.0) | 90.7 (2.2)b |
The cell density is defined as the mean number of cells per mm2. Percentages of single-positive (e.g., % CD3+) cells in relation to all cells and the proportions of “conventional” cells with respect to the total number of positive cells (e.g., % CD3+CD68−/CD3+). Hybrid cells with co-expression of CD68 were considered “disturbed”. The data are presented as mean (SE). Significant differences between zones according to Mann–Whitney U test: azone1 and zone2, bzone1 and zone6