Fig. 2.

Pacs2 is decreased upon Chop overexpression. A MLE12 cells were either not induced or were induced with 1 µg/ml doxycycline for Chop expression for 12 and 24 h, followed by immunoblots for the given proteins from total lysates. B Quantification of significant decrease in Pacs2 protein levels upon Chop induction. Relative protein amounts were normalized to Gapdh and their level in −dox cells was set as one. C Representative immunoflourescence images of Pacs2 (green) in −dox or + dox-treated cells for 24 h. Nuclei were stained with DAPI (blue), scale bar = 60 µm. D Fluorescence intensity was quantified using ImageJ, and its intensity in −dox cells was set to one. Blots and analysis were performed from n = 3 experiments and statistical significance is indicated as: **p ≤ 0.01