Fig. 3.

Pacs2 overexpression rescues phenotype of Chop-induced cells. MLE12 cells were either mock transfected, or transfected with empty-Myc or Myc-PACS2 for 4 h, followed by no dox (− dox) or with dox (+ dox, 12 h) treatment for Chop overexpression, followed by (A) proximity ligation assay with antibodies against calnexin and VDAC1 and subsequent fluorescence microscopy, scale bar = 60 µm. B Fluorescence intensity was quantified using ImageJ, its intensity in untreated −dox cells was set to one. C Immunoblots for the indicated proteins. D Relative protein levels of Pacs2 and E cleaved caspase 3 were normalized to Gapdh and their level in −dox cells was set as one. Representative images and stainings from n of 3 independent experiments are shown, statistical significance is indicated as: *p ≤ 0.05, ***p ≤ 0.001