Nelfb+/+Villin-Cre and Nelfbfl/flVillin-Cre mice were treated with 3 or 4% DSS in drinking water for 5 days. a–c Body weights (a), survival (b) (n = 24–28) and colon length (c) (n = 3–4) of Nelfb+/+Villin-Cre and Nelfbfl/flVillin-Cre mice after DSS treatment. d, e H&E staining (d) and histological scores (e) (n = 4) of colon sections from DSS treated mice at day 4. Scale bar, 50 μm. f, g H&E staining (f) and histological scores (g) (n = 4) of colon sections from DSS treated mice at day 8. Scale bar, 50 μm. Data are pooled from more than 3 (a, b) independent experiments and shown as mean ± SEM. Student’s t-test was performed; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Images represent at least two independent experiments (c, d, and f).