Figure 4.
Regulation of polyamine levels by Spd supplementation during experimental CAC. (A) Putrescine, spermidine, and spermine concentrations were measured by LC/MS in non-tumor (NT) and tumor (T) tissues from WT and Smox–/– mice ± AOM-DSS ± Spd. (B) Smox mRNA expression was assessed by RT-real-time PCR. In A and B, *P < .05, **P < .01, ***P < .001, ****P < .0001 by two-way ANOVA and Tukey test. (C) Correlation plots comparing the average tumor size of WT mice not receiving Spd supplementation to the corresponding polyamine concentration; this figure includes the animals depicted in A plus data from additional WT mice not treated with Spd. The Pearson r and the P values were determined by simple linear regression.