figs4.

Immunogold cytochemical control of anti-dsRNA antibody in non-infected Vero E6 cells, with and without plitidepsin. Ultrathin sections of cells were incubated with anti-dsRNA primary antibody followed by a secondary antibody conjugated with 10 nm colloidal gold particles and visualized by TEM. Low (A) and high (B) and (C) magnification images. (D) to (F) Vero E6 cells treated 48 h with 0.05 μM plitidepsin. Low (D) and high (E) and (F) magnification images. (G) to (I) Vero E6 cells treated 48 h with 0.2 μM plitidepsin. Low (G) and high (H) and (I) magnification images. Cells present no unspecific background. N, nucleus; P, plasma membrane; M, mitochondrion. Scale bars, 1 μm in A, D and G; 200 nm in B, C, E, F, H and I.