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. 2022 Feb 25;8:83. doi: 10.1038/s41420-022-00878-w

Fig. 6. Effect of KIAA1429 on regulating SIRT1 mRNA stability.

Fig. 6

A SW480 and HCT116 cell lysates were immunoprecipitated with KIAA1429 antibody followed by QRT-PCR measuring transcript levels of all indicated targets. B PCR measured transcript levels of SIRT1 and GAPDH within KIAA1429 or IgG immunocomplexes in SW480 and HCT116 cell lysates. C SW480 and HCT116 cells were infected with scr and KIAA1429 shRNA, the cell lysates were immunoprecipitated with m6A antibody followed by QRT-PCR measuring transcript levels of SIRT1. *P < 0.05, **P < 0.01. D SW480 and HCT116 cells were infected with scr and KIAA1429 shRNA for 72 h, then treated with 8 μg/ml Act D for 0, 1, 2, 4, 6, 8 h. The stability of SIRT1 mRNA was described in Materials and Methods. **P < 0.01.