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. 2022 Feb 25;13:1056. doi: 10.1038/s41467-022-28591-3

Fig. 2. Chemogenetic inhibition of neural firing in the PFC.

Fig. 2

a Experimental design of chemo-fMRI experiments. AAV8-hSyn-hM4Di (n = 15) or AAV8-hSyn-GFP (control, n = 19) were bilaterally injected into the PFC of wild type. Left: representative histology sample showed hM4Di (red) expression. Right: heatmaps illustrate a qualitative regional assessment of viral expression across subjects. b Mice underwent chemo-fMRI scanning or c electrophysiological recordings to probe effectiveness of chemogenetic manipulations. A reference acquisition timeline is reported to depict timeseries binning into a 15-min pre-CNO reference baseline, a drug equilibration window (15 min, transition), and a 35-min CNO active time window (active). d Representative raw traces collected before and after CNO injection in representative recordings site of a hM4Di-expressing mouse. e, f Reduced firing rate in hM4Di-expressing mice (n = 5) compared to GFP-transduced controls (n = 5, two-sided Wilcoxon rank-sum tests, FDR corrected **q < 0.01, ***q < 0.001). Data are presented as mean values ± SEM. g Scatterplot comparing the firing rate of individual PFC recording channels during baseline conditions (x axis) and the active phase (y axis) in control and DREADD-expressing animals (two-sided Wilcoxon rank-sum test FDR corrected, **q < 0.01, ***q < 0.001). Source data are provided as a Source Data file.