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. 2022 Feb 25;13:1058. doi: 10.1038/s41467-022-28609-w

Fig. 4. Unlike DAGLB, DAGLA is not rerouted in an AP-4 cargo redistribution assay.

Fig. 4

a Widefield imaging of wild-type HeLa or HeLa cells stably expressing GFP-RUSC2 (blue), transiently expressing HA-DAGLB, and labelled with anti-HA (green) and anti-ATG9A (red). HA-DAGLB has a very similar localisation to ATG9A in both cell lines. The insets show the rerouting of HA-DAGLB to peripheral ATG9A- and RUSC2-positive puncta in cells overexpressing GFP-RUSC2. Scale bar: 10 µm. b Widefield imaging of wild-type HeLa or HeLa cells stably expressing GFP-RUSC2, transiently expressing HA-DAGLA, as in (a). HA-DAGLA has a different localisation to ATG9A and does not reroute to the peripheral puncta in cells overexpressing GFP-RUSC2. Scale bar: 10 µm. For (a) and (b), images are representative of at least 18 images per condition, including three independent transfections with microscopy performed independently for each.