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. 2022 Feb 25;12:3213. doi: 10.1038/s41598-022-07131-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Research flowchart. The upper part of figure (a) shows the six dogs included in the NGS SAR Trial. The serum of six dogs was collected at two experimental time points (T0 and T1), RNA was extracted and processed for next-generation sequencing miRNome. The figure below (b) shows the second part of the research plan referred to as QPCR SAR Cluster. This part of the study includes blood dog sampling at T0 and T1 of the 2nd, 3rd, and 4th SAR trials. Serum RNA was extracted, reverse transcribed, and only the microRNAs differently expressed, detected in NGS SAR Trial, were validated by qPCR. The figure is created by G. Guelfi using PowerPoint 2021, Microsoft Corporation, USA.