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. 2022 Feb 25;12:3213. doi: 10.1038/s41598-022-07131-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Volcano plot. The figure shows the relationship between the magnitude of the difference in expression values (fold change) and statistical p-values. The lower difference in expression, the nearer the point will be to the x-axis. The more significant the difference, the smaller the p-value, and the higher -log₁₀ p-value. Thus, points for features with highly significant differences will lie high in the plot. The cfa-let-7f (0.000002 p-value) and cfa-let-7a in the plot (red) represent the differentially expressed ecmiRNAs with statistical significance (0.000253 p-value).