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. 2022 Feb 25;12:3213. doi: 10.1038/s41598-022-07131-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EcmiRNAs expression levels. The normalized expression values (y-axis) of the let-7a and let-7f ecmiRNAs were calculated with the 2^-ΔCq. The let-7a and let-7f graph show a white box (T0; 22 dog serum samples) and a grey box (T1; 22 dog serum samples). In the box plots, the thick central line represents the median; the top and bottom lines of the box represent the third quartile and the first quartile; whiskers indicate the variability in the data outside the upper and lower quartile. The figure shows the statistical significance of the overlap between the two groups determined using a two-tailed Student’s t-test; ***p ≤ 0.001.