Table 1.
List of primers used in qPCR.
| Primer | Recommended for: | QIAGEN ID |
|---|---|---|
| UniSp2 | Spike-in (RNA isolation efficiency assessment) | YP00203950 |
| UniSp4 | Spike-in (RNA Isolation efficiency assessment) | YP00203953 |
| UniSp6 | Spike-in (RT and PCR inhibitors assessment) | YP00203954 |
| cfa-miR-320 | Serum EC (qPCR normalized quantities) | YP00206042 |
| cfa-miR-148a | Serum EC (qPCR normalized quantities) | YP00205867 |
| cfa-miR-24 | Serum EC (qPCR normalized quantities) | YP02114589 |
| cfa-miR-23a | Serum EC (qPCR normalized quantities) | YP00205956 |
| cfa-let-7a | Target miRNA validation | YP00205727 |
| cfa-let-7f | Target miRNA validation | YP00204359 |
The resuspended primer mix contains a forward and reverse recognition sequence. The table reported Spike-in primers, serum EC, and differential target miRNA selected by NGS.