Table 1.
Comparison of mean ELISA A450 signals in serum and vitreous fluid.
| Measurements | Charlesworth et al.4 (Serum) | Present study (Paired serum) | Present study (Paired vitreous fluid) | Present study (Validation vitreous fluid) |
|---|---|---|---|---|
| Sample size | 125 | 13 | 13 | 36 |
| α-Tetanus positive samples | 99% | 100% (13/13) | 100% (13/13) | 92% (33/36) |
| α-Albumin positive samples | 0.8% | 0% (0/13) | 0% (0/13) | 3% (1/36) |
| α-Hemoglobin positive samples | Not shown | 0% (0/13) | 0% (0/13) | 3% (1/36) |
| α-SaCas9 positive samples | 78% | 100% (13/13) | 15% (2/13) | 0% (0/36) |
| α-SpCas9 positive samples | 58% | 100% (13/13) | 15% (2/13) | 3% (1/36) |
| α-Tetanus mean A450 | 2.057 | 3.162 | 1.070 | 0.640 |
| α-Hemoglobin mean A450 | Not shown | 0.125 | 0.072 | 0.074 |
| α-Albumin mean A450 | 0.340 | 0.081 | 0.054 | 0.063 |
| α-SaCas9 mean A450 | 1.340 | 0.500 | 0.065 | 0.061 |
| α-SpCas9 mean A450 | 1.092 | 0.602 | 0.073 | 0.069 |
| Threshold cutoff for positivity (Mean α-Albumin or α-Hemoglobin + 3 s.d.) | 0.879 (α-Albumin) | 0.195 (α-Hemoglobin) | 0.088 (α-Hemoglobin) | 0.107 (α-Hemoglobin) |