Figure 7.

PDGF-BB increases cell migration in vitro. Effect of PDGF-BB on wound scratch assay. Confluent human PDL fibroblasts were subjected to injury by scratching with a plastic pipette tip. The cells were then cultured under 0.3% FBS (solvent, (a–c)), PDGF-BB (5 ng/mL, (d–f)), Wnt3a (25 ng/mL, (g–i)), or their combination (P&W, (j–l)). Pictures were taken at 0 h (T₀; (a,d,j,g)), 24 h (T₂₄; (b,e,h,k)), and 48 h (T₄₈; (c,f,i,l)). (e) Ratios (T₂₄/T₀) and (T₄₈/T₀) of wound area at T₂₄ and that at T₄₈ to original wound area at T₀. Mean ± 1 SD are shown. Significant differences between two groups, *p < 0.05, **p < 0.01, ***p < 0.01 (Fisher PLSD method).