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. 2022 Feb 25;42(2):BSR20212241. doi: 10.1042/BSR20212241

Figure 2. miR-29b-3p targeted VEGFA in vitro.

Figure 2

(A) The binding sites between miR-29b-3p and the 3′UTR of VEGFA. (B) RT-PCR tested the expression of miR-29b-3p following 24 h of cell transfection with miR-29b-3p mimics and miR-29b-3p inhibitor into HEK 293T cells. (C) A target gene of interest is transfected with a luc gene that expresses the enzyme luciferase at the target site, and the luciferase activity of VEGFA-WT was tested following 48 h of cell con-transfection (x ± s, n=3, **P<0.05, ***P<0.01, ****P<0.001).