Figure 10.

Enhanced expression of ID2 in Tob1^–/–CD4⁺T cells restrains Th1/Th17 cell immune responses. Splenic CD4⁺ T cells were harvested from Tob1^–/– mice (n = 6), transfected with LV-Id2 and LV-NC, respectively, and then cultured under stimulation with plate-bound anti-CD3 mAb (5 μg/mL) and soluble anti-CD28 mAb (2 μg/mL) for 5 days. (A) The transfection efficiency of LV-Id2 was confirmed by qRT-PCR, and (B–H) the mRNA expression of Il-17a, Rorγt, Ifn-γ, T-bet, Tnf-α, Il-10, and Foxp3 in these CD4⁺ T cells was detected by qRT-PCR. (I–L) The culture supernatants of these transfected CD4⁺ T cells were also collected for detecting the levels of IFN-γ, IL-17A, TNF-α, and IL-10, respectively, by ELISA. Statistical analysis was performed with unpaired 2-sided Student’s t tests. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, and ∗∗∗∗P < .0001 vs LV-NC from the same group.