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. 2021 Dec 14;13(4):1201–1221. doi: 10.1016/j.jcmgh.2021.12.007

Figure 9.

Figure 9

Enhanced expression of ID2 in IBD CD4+T cells confines Th1/Th17 cell immune responses. PB-CD4+ T cells were harvested from patients with A-CD (n = 8), patients with A-UC (n = 8), and HC subjects (n = 8), transfected with LV-ID2, LV-shID2, and LV-NC, respectively, and then cultured under stimulation with plate-bound anti-human CD3 mAb (5 μg/mL) and soluble anti-human CD28 mAb (2 μg/mL) for 5 days. (A) The transfected CD4+ T cells were harvested on day 5, and transfection efficiencies of LV-ID2 and LV-shID2 were confirmed by qRT-PCR. (B–H) qRT-PCR was performed to examine the mRNA expression of IFN-γ, IL-17A, TNF-α, T-bet, RORC, Foxp3, and IL-10 in these CD4+ T cells. (I–L) The culture supernatants of these transfected CD4+ T cells were also collected for detecting the levels of IFN-γ, IL-17A, TNF-α and IL-10 using ELISA. Statistical analysis was performed with unpaired 2-sided Student’s t tests. ∗P < .05, and ∗∗P < .01 vs LV-NC from the same group.