Table II.
Effect of ROI scavengers and related inhibitors on O·2− release by radish seed coats
| Inhibitor | O·2− Release |
|---|---|
| % | |
| None | 100 ± 5 |
| Superoxide dismutase (100 μg mL−1) | 59 ± 8 |
| Mn-desferal (300 μm) | 0 ± 0 |
| Diphenyleneiodonium (DPI) (100 μm)a | 48 ± 14 |
| KCN (1 mm) | 97 ± 14 |
| NaN3 (1 mm) | 89 ± 7 |
| Catalase (100 μg mL−1) | 82 ± 21 |
| Na-benzoate (10 mm) | 82 ± 21 |
| Adenine (5 mm) | 123 ± 15 |
| Mannitol (10 mm) | 81 ± 14 |
| Na-formate (10 mm) | 153 ± 24 |
| Thiourea (10 mm) | 91 ± 20 |
| Urea (100 mm) | 115 ± 14 |
The O·2−-dependent reduction of XTT by 20 coats from 48-h germinated seeds during a 3-h incubation period was determined in the presence of various inhibitors.
a
Including 10 μL mL−1 dimethylsulfoxide (ineffective in controls).